| Educational guide Faculty of Chemistry |
english |
| Bachelor's Degree in Biochemistry and Molecular Biology (2009) |
 Subjects |
| METHODOLOGY AND EXPERIMENTATION IN MOLECULAR BIOSCIENCES I |
| Contents |
| IDENTIFYING DATA | 2019_20 |
| Subject | METHODOLOGY AND EXPERIMENTATION IN MOLECULAR BIOSCIENCES I | Code | 13214121 | |||||
| Study programme |
|
Cycle | 1st | |||||
| Descriptors | Credits | Type | Year | Period | ||||
| 3 | Compulsory | Third | 1Q |
|||||
| Competences | Learning outcomes | Contents |
| Planning | Methodologies | Personalized attention |
| Assessment | Sources of information | Recommendations |
| Topic | Sub-topic |
| Cloning genes of Lambda phage DNA in E. coli | 1. Restriction of the Lambda phage DNA 2. Linking restriction fragments with the pUC19 vector 3. Transforming E. coli with the ligation mixture and selecting transformed bacteria 4. Identifying colonies of E. coli carriers of recombinant plasmid by color (white / blue) 5. Minipreparation of plasmid DNA from E. coli 6. Checking the fragments and genes of the Lambda beam cloned to pUC19 by restriction analysis |
