| Educational guide Faculty of Chemistry |
english |
| Bachelor's Degree in Biochemistry and Molecular Biology (2009) |
 Subjects |
| MOLECULAR GENETICS AND GENETIC ENGINEERING |
| Contents |
| IDENTIFYING DATA | 2019_20 |
| Subject | MOLECULAR GENETICS AND GENETIC ENGINEERING | Code | 13214112 | |||||
| Study programme |
|
Cycle | 1st | |||||
| Descriptors | Credits | Type | Year | Period | ||||
| 6 | Compulsory | Third | 1Q |
|||||
| Competences | Learning outcomes | Contents |
| Planning | Methodologies | Personalized attention |
| Assessment | Sources of information | Recommendations |
| Topic | Sub-topic |
| 1. Introduction. | Organization of the subject. Basic concepts of Molecular Genetics and Genetic Engineering. |
| 2. Manipulation and characterization of DNA and RNA in vitro. | Chemical synthesis of DNA and RNA. DNA and RNA polymerases. PCR. DNA and RNA sequencing. Restriction enzymes. Ligases. DNA marking. Southern, Northern. DNA microarrays. |
| 3. Molecular Cloning in Escherichia coli. | Vectors for the amplification and expression of genes in E. coli: plasmids and bacteriophages. Use of DNA in E. coli recombinant selection. Modification and expression of cloned genes. Genomic library and expression libraries. Expression of recombinant proteins in E. coli. |
| 4. Genetic engineering in Saccharomyces cerevisiae yeast. | |
| 5. Classical genetic engineering in plants. | |
| 6. Classical genetic engineering in animal cells and organisms. | |
| 7. Editing eukaryotic genomes using CRISPR / Cas9 technology. | |
| 8. Manipulating gene expression in eukaryotes using small silencing RNAs. | |
| 9. Pluripotent and induced pluripotent stem cells. | |
| 10. Organizing the genome in model eukaryotic organisms. | |
| 11. Recombination in eukaryotic genomes. | |
| 12. Genetic variations and genetic diseases. |
