| Topic |
Sub-topic |
| Session 1. Introduction to animal cell cultures. |
Brief history of cell cultures. Current concept, applications and research areas. Type of tissue cultures. Biology of the cell in culture. |
| Session 2. The cell culture laboratory. |
Equipment and design. Levels of biosecurity. Classification of biological agents. Concept of SDS, PPEs and SLP. |
| Session 3. Physical-chemical conditions, media and cultivation surfaces. |
Temperature, pH and osmolarity. Composition, preparation, selection and optimization of culture media. Adaptation to serum-free media. Cell growth surfaces. |
| Session 4. Biological contamination. |
Aseptic technique. Main biological pollutants: bacteria, yeasts, fungi, viruses and mycoplasmas. Detection procedures and methods. |
| Session 5. Quantification, cytotoxicity and cell death. |
Neubauer chamber. Cell viability and cytotoxicity assays. |
| Session 6. Introduction to flow cytometry. |
Brief history. What information does the CMF provide us? Practical aspects. FACS |
| Session 7. Basic techniques of manipulation of animal cells in culture. |
Change of medium. Pass or subculture. Cryopreservation. Transformation and immortality. Transport of cells. Cell characterization. |
| Session 8. Specialized cell cultures and associated advanced techniques. |
Cell isolation techniques. Cultivation examples. Cell separation techniques. Cell lines by biotechnology. |
| Session 9. Cellular modification systems. |
Genetic marker. DNA introduction techniques in animal cells in culture. Animal cells as factories of production: drugs, proteins and antibodies. Protein introduction techniques in animal cells in culture. |
| Session 10. Biotechnology of animal and tissue cells. |
Stem cells vs. culture of specialized cells. Isolation and culture technology of stem cells. Cell differentiation techniques. Reconstruction of tissues and organs by co-culture of primary type. |
Subjects
