2009_10
Guia docent 
Escola Tècnica Superior d'Enginyeria Química
A A A 
català 
Nanociència i Nanotecnologia (2006)
 Assignatures
  BIOQUÍMICA A LA NANOESCALA
   Continguts
Tema Subtema
1- General view of Biochemistry. Cellular, chemical and genetic fundamentals of live. Water. Glycids. Lipids. Nucleic acids.
2- Aminoacides, peptides and proteins. Amino acides. Peptide bond and primary structure. Three-dimensional structure of proteins. Folding and denaturalization.
3- Methods to study proteins. Purification and characterization. Determination of amino acidic sequence. Mass spectrometry. Determination of three-dimensional structure; X ray diffraction and nuclear magnetic resonance.
4- Enzymes. Enzymatic catalysis. Michaelis-Menten model. Allosteric enzymes. Enzymatic inhibition. Regulation of enzymatic activity. Catalytic strategies. General view of metabolism.
5- Biomembranes. Lipids as a biomembrane components. Proteins as a biomembrane components. Transport and energy transduction. Examples of biomembranes components: membrane transporters, potassium-sodium pump, ATPases, TMS transporters.
6- Nucleic acids. Composition and structure of DNA and RNA. Physic and chemical properties. Interactions between proteins and nucleic acids. Special nucleotides: TFOs, TFBs. Hairpins, aODNs, iRNAs, biological applications.
7- Methods to study nucleic acids. Electrophoresis. Nucleic acid hybridisation. Enzyme to handle nucleic acids. Polymerase chain reaction (PCR): fundamentals and applications. DNA sequencing. DNA cloning.
8- Micro arrays. Types. Construction: lithographic techniques. Applications: gene expression determination, genotyping, SNPs identification, specific binding to transcription factors, identification of protein interactions.