| Educational guide Faculty of Chemistry |
english |
| Nutrition and Metabolism (2012) |
 Subjects |
| TRAINING COURSE IN ANIMAL EXPERIMENTATION |
| Contents |
| IDENTIFYING DATA | 2021_22 |
| Subject | TRAINING COURSE IN ANIMAL EXPERIMENTATION | Code | 13675204 | |||||
| Study programme |
|
Cycle | 2nd | |||||
| Descriptors | Credits | Type | Year | Period | ||||
| 3 | Optional | 2Q |
||||||
| Competences | Learning aims | Contents |
| Planning | Methodologies | Personalized attention |
| Assessment | Sources of information | Recommendations |
| Topic | Sub-topic |
| Topic 1. Introduction to laboratory animal science | 1.1. History of animal experimentation. |
| Topic 2. Legislation. | 2.1. European, national and regional legal framework. Ethical committees. 2.2. Authorizations: authorization forms, application forms to acquire animals. |
| Topic 3. Ethics and animal welfare | 3.1. General principles. The 3 R’s. 3.2. Ethics and responsibilities of people working with experimental animals. 3.3. Concept of damage and avoidable damage. The five freedoms of animals. 3.4. Severity classification system of a procedure. 3.5. Regulations for the reuse of animals 3.6. Information sources. |
| Topic 4. Care, health and handling of animals (level 1 and 2) | 4.1. Transport, installations and environmental conditions for location of animals. 4.2. Nutrition and food: nutritional requirements, types of diets. 4.3. Microbiology and diseases of laboratory animals. Influence on research. 4.4. Factors that influence behaviour. Homeostasis and stress. |
| Topic 5. Biology of animals used in experimentation. |
5.1. Anatomy and physiology compared. Rodents (rat/mouse). 5.1.1. Rats and mice: non consanguineous and consanguineous. 5.1.3. Animal models: congenics, hybrids, transgenic, knock-outs and others. 5.2. Situations that can cause pain and / or stress. 5.3. Reproductive characteristics in rats and mice. 5.3.3. Pregnancy, birthing and lactation. Indices of viability and lactation. |
| Topic 6. Recognition of pain, suffering and distress | 6.1. Severity index, endpoint criteria, and animal welfare monitoring. |
| Topic 7. Euthanasia (level 1 and 2) | 7.1. Euthanasia methods. 7.2. Euthanasia in conscious and unconscious animals. |
| Topic 8. lternative methods to the use of laboratory animals. | 8.1. In vitro and cell/tissue culture models. 8.2. Alternative techniques in pharmacology and toxicology. |
| Topic 9. Minimally invasive procedures (level 1, theoretical; level 2, practical) | 9.1. Handling, immobilization (rat/mouse). 9.2. Administration of substances (routes of administration) and sampling (collection of blood, urine and faeces). 9.2.1. Routes of treatment administration (oral intragastric, rectal and parenteral). 9.2.2. Obtaining blood samples. Precautions and requirements to consider when obtaining blood samples. 9.2.3. Obtaining urine and faeces samples. 9.2.4. Conditions for obtaining and processing biological samples. |
| Topic 10. Anaesthesia | 10.1. Concept of anaesthesia 10.1.1. Choice of anaesthetic technique 10.1.2. Phases of the anaesthetic technique 10.1.3. Supportive measures during anaesthesia 10.1.4. Anaesthetic recovery 10.2. Analgesia: recognition and evaluation of pain 10.2.1. Analgesia techniques 10.3. Euthanasia in unconscious animals |
| Topic 11. Principles of surgery: minor procedures and prolonged surgical procedures | 11.1. Basic concepts in small animal surgery 11.2. Feeding protocol, fasting and anaesthesia 11.3. Basic pre and postoperative care 11.4. Identification of signs of animal suffering 11.5. Nomenclature and manipulation of basic surgical instruments 11.6. Blood extraction methods: tail, jugular, intracardiac 11.7. Recognition of internal structures of the animal: medium laparotomy and closure by planes 11.8. Tissue biopsy methods 11.9. Euthanasia in surgery |
| Topic 12. Design of projects and procedures (level 1 and 2) | |
| Topic 13. Introduction to institutional organization |
