2022_23
Educational guide 
Faculty of Oenology
A A 
english 
Bachelor's Degree in Biotechnology (2009)
 Subjects
  ENZYMOLOGY
   Contents
Topic Sub-topic
1. Introduction Enzyme definition. Historical perspective. Main features of enzymes. Cofactors. Nomenclature and classification of enzymes.
2. Enzymatic kinetics Definition of reaction rate. Measurement of enzymatic activity. Enzymatic kinetics of the reactions of a substrate. Enzymatic inhibition in the reactions of a substrate. Effects of pH and temperature. Enzymatic kinetics of the reactions of two or more substrates.
3. Enzymatic purification Objectives. Purification strategies. Methods of separation. Follow-up purification. Examples of purification.
4. Introduction to Mechanisms of enzymatic action Bases of catalysis. Examples of enzymatic mechanisms. Ribozimes.
5. Regulation of enzyme activity Mechanisms of regulation of enzymatic activity. Cooperativity and allosterism.