| Educational guide School of Chemical Engineering |
english |
| Nanoscience and Nanotechnology (2006) |
 Subjects |
| NANOSCALE BIOCHEMISTRY |
| Contents |
| IDENTIFYING DATA | 2010_11 |
| Subject | NANOSCALE BIOCHEMISTRY | Code | 205151104 | |||||
| Study programme |
|
Cycle | 2nd | |||||
| Descriptors | Credits | Type | Year | Period | ||||
| 5 | Compulsory | First | Only annual |
|||||
| Competences | Learning aims | Contents |
| Planning | Methodologies | Personalized attention |
| Assessment | Sources of information | Recommendations |
| Topic | Sub-topic |
| 1- General view of Biochemistry. | Cellular, chemical and genetic fundamentals of live. Water. Glycids. Lipids. Nucleic acids. |
| 2- Aminoacides, peptides and proteins. | Amino acides. Peptide bond and primary structure. Three-dimensional structure of proteins. Folding and denaturalization. |
| 3- Methods to study proteins. | Purification and characterization. Determination of amino acidic sequence. Mass spectrometry. Determination of three-dimensional structure; X ray diffraction and nuclear magnetic resonance. |
| 4- Enzymes. | Enzymatic catalysis. Michaelis-Menten model. Allosteric enzymes. Enzymatic inhibition. Regulation of enzymatic activity. Catalytic strategies. General view of metabolism. |
| 5- Biomembranes. | Lipids as a biomembrane components. Proteins as a biomembrane components. Transport and energy transduction. Examples of biomembranes components: membrane transporters, potassium-sodium pump, ATPases, TMS transporters. |
| 6- Nucleic acids. | Composition and structure of DNA and RNA. Physic and chemical properties. Interactions between proteins and nucleic acids. Special nucleotides: TFOs, TFBs. Hairpins, aODNs, iRNAs, biological applications. |
| 7- Methods to study nucleic acids. | Electrophoresis. Nucleic acid hybridisation. Enzyme to handle nucleic acids. Polymerase chain reaction (PCR): fundamentals and applications. DNA sequencing. DNA cloning. |
| 8- Micro arrays. | Types. Construction: lithographic techniques. Applications: gene expression determination, genotyping, SNPs identification, specific binding to transcription factors, identification of protein interactions. |
