2016_17
Guia docent 
Facultat d'Enologia
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Grau de Biotecnologia (2009)
 Assignatures
  BIOTECNOLOGIA MOLECULAR ANIMAL I VEGETAL
   Continguts
Tema Subtema
VEGETAL TEMA 1. Introducció a la biotecnologia vegetal Definicions
Dades de producció
Evolució de la millora vegetal
Aplicacions
VEGETAL TEMA 2. Desenvolupament dels vegetals Teixits de les plantes
Definicions
Control del cicle cel·lular vegetal
Embriogènesi
Meristems
VEGETAL TEMA 3.Cultius cel·lulars vegetals Cultius in vitro vegetals
Etapes
Aplicacions
Explants
VEGETAL TEMA 4.Variació somaclonal Definició
Factors
Avantatges i inconvenients
Mètodes de detecció
VEGETAL TEMA 5. Agrobacterium tumefaciens Definició
Plàsmid Ti
Mecanisme d'actuació
Aplicació en engenyeria genètica
VEGETAL TEMA 6. Tècniques de transferència gènica en vegetals Limitacionss d' Agrobacterium
Tècnicas de transferència directa
Transformació de protoplasts
Biolística
Electroporació
Microinyecció
Whiskers
Animal Topic 1
Introduction to animal biotechnology
 Definition and objectives
Historical facts
The technologies involved
Applications
Regulation and concerns
Animal Topic 2
Animals as laboratory tools
 Natural and laboratory populations
Key principles of 3Rs
Mouse as model system
Genetic standardization
Inbred strains, F1 hybrids, coisogenic, congenic and outbred strains among others
Mouse nomenclature
Genetic drift and substrain development
Genetic integrity monitoring and SNPs
Cryopreservation
Animal Topic 3
Early embryogenesis and reproductive biology in laboratory mouse
 Preformism versus epigenesis
Stages of embryogenesis
Spermatogenesis
Oogenesis
Fertilization
Acrosomal and cortical reaction
Ca flux
Zona pellucida
Segmentation and gastrulation
Morula and blastula
Ectoderm, endoderm and mesoderm
Organogenesis
Reproductive characteristics
Sex determination
Male anatomy and sperm production
Female anatomy and egg production
Estrous cycle
Mating options
Problems
Animal Topic 4
Assisted reproduction technologies (ARTs) in human and animals
 Assisted reproduction technologies (ARTs)
Human infertility
Causes of female and male infertility
Controled ovarian hyperstimulation (COH) and artificial insemination (AI)
Cryopreservation- egg, sperm and embryo banking
Sex determination
In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI)
Preimplantation genetic diagnostics (PGD)
Animal Topic 5
Cloning 		Reproductive cloning
Embryos and biotechnology
Reproductive and therapeutic cloning
History of cloning
Embryo splitting
Somatic cell nuclear transfer (SCNT) Dolly
Problems and difficulties
Cloning of endangered and extinct species
Therapeutic cloning
Regenerative medicine and therapeutic cloning
Stem cells
Adult stem cells
Embryonic stem (ES) cells
Somatic cell nuclear transfer (SCNT) stem cells
Induced pluripotent stem cells (iPS cells)
Stem cell-based therapies
Animal Topic 6
Non-targeted transgenesis
 Physiological, pathological and induced models
Forward versus reverse genetics
Non-targeted transgenesis
Pronuclear injection (PNI)
Viral transgenesis
Sperm-mediated transgenesis
Animal Topic 7
Targeted transgenesis
 Gene targeting
Mouse embryonic stem (ES) cells
Homologous recombination
Positive and negative selection of ES cell clones
Typical gene targeting experiment
Blastocyst injection
Chimera production and breeding
YAC transgenes
Knock out (KO) and Knock in (KI)
Conditional strategies
Cre/loxP recombination system
Flp/Frt system
Inducible strategies
Cre-ERT system
TetOn/TetOff systems
Reporter mouse
RNAi
Animal Topic 8 Applications in transgenic livestock
 Transgenic livestock
Methodologies
Pharming (biopharming)
Applications in agriculture: enhanced nutrition, reduced environmental impact, enhanced milk,
enhanced growth rates and carcass composition, improved disease resistance, improved reproductive performance and fecundity, improved hair and fiber
Risks and conclusions
Animal Topic 9 Transgenesis and pathology
 Non-targeted transgenesis and HER2 TG mice in breast cancer
Targeted transgenesis, colon stem cells and colon cancer
Aging
Hutchinson-Gilford progeria syndrome (HGPS)